Interactive comment on “Activity and abundance of denitrifying bacteria in the subsurface biosphere of diffuse hydrothermal vents of the Juan de Fuca Ridge” by A. Bourbonnais et al
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Reviewer’s comment: The paper is overall well written, my only argument is for the bacterial community analysis and q-PCR. I am afraid that the 16S rRNA gene library analysis performed in this study is weak. Only very limited clones were sequenced from the five bacterial 16S rDNA clone libraries, for example, only 27 clones from library of Phang were sequenced, thus the coverages of the libraries were low, mostly around 50-60%. Therefore, the compositional analysis of different bacterial groups in the environments were not convincing at all. I understand that one of the main focuses of this paper is to determine the relative contributions of different Nitrogen elimination
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Interactive comment on “Activity and abundance of denitrifying bacteria in the subsurface biosphere of diffuse hydrothermal vents of the Juan de Fuca Ridge” by A. Bourbonnais et al
Reviewer’s comment: My main criticism of the manuscript is that data about the abundance of denitrifiers are excluded from the manuscript, even though we are told they exist. Why are the nirS and nirK data separated from this manuscript? Are those data really so abundant as to warrant their own manuscript? Being able to add quantitative information about the entire denitrifying community would ...
متن کاملInteractive comment on “Activity and abundance of denitrifying bacteria in the subsurface biosphere of diffuse hydrothermal vents of the Juan de Fuca Ridge” by A. Bourbonnais et al
This is a nice paper presenting their data on nitrogen transformation in particular nitrogen loss at deep-sea diffuse vents. Through their data, they could determine the potential rates of denitrification, annamox, and DNRA using 15N paired isotope techniques. Their result suggested that bacterial denitrification is the dominant nitrogen elimination process in sulfidic hydrothermal vent waters....
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